Fluorescein staining is highlighted by the use of filters. The biomicroscope has a cobalt (blue) filter which can be placed in the light path. A Wratten 47B filter can also be used. The blue filter enhances the staining. The staining can be further highlighted by placing a yellow filter (Wratten #12 or Tiffin #2) over the objective of the biomicroscope. The top photo shows a yellow filter sliding into the filter holder over the objective. The bottom photo shows the filter as it comes from the photoshop.
